Depth-resolved multimodal imaging: Wavelength Modulated Spatially Offset Raman Spectroscopy with Optical Coherence Tomography

Jonathan Nylk
Tuesday 11 July 2017

Mingzhou Chen, Josep Mas, Lindsay H. Forbes, Melissa R. Andrews, and Kishan Dholakia

Journal of Biophotonics, 10.1002/jbio.201700129 (2017)

doi: 10.1002/jbio.201700129

A major challenge in biophotonics is multimodal imaging to obtain both morphological and molecular information at depth. We demonstrate a hybrid approach integrating optical coherence tomography (OCT) with wavelength modulated spatially offset Raman spectroscopy (WM-SORS). With depth co-localization obtained from the OCT, we can penetrate 1.2mm deep into the strong scattering media (lard) to acquire up to a 14-fold enhancement of a Raman signal from a hidden target (Polystyrene) with a spatial offset.

Our approach is capable of detecting both Raman and OCT signals for pharmaceutical particles embedded in turbid media and revealing the white matter at depth within a 0.6mm thick brain tissue layer. This depth resolved label-free multimodal approach is a powerful route to analyze complex biomedical samples.

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