Integrated single- and two-photon light sheet microscopy using accelerating beams

Jonathan Nylk
Friday 12 May 2017

Peeter Piksarv, Dominik Marti, Tuan Le, Angelika Unterhuber, Lindsey H. Forbes, Melissa R. Andrews, Andreas Stingl, Wolfgang Drexler, Peter E. Andersen & Kishan Dholakia

Scientific Reports 7, 1435 (2017)

doi: 10.1038/s41598-017-01543-4

We demonstrate the first light sheet microscope using propagation invariant, accelerating Airy beams that operates both in single- and two-photon modes. The use of the Airy beam permits us to develop an ultra compact, high resolution light sheet system without beam scanning. In two-photon mode, an increase in the field of view over the use of a standard Gaussian beam by a factor of six is demonstrated. This implementation for light sheet microscopy opens up new possibilities across a wide range of biomedical applications, especially for the study of neuronal processes.

Related topics

Share this story