Enhancement of image quality and imaging depth with Airy light-sheet microscopy in cleared and non-cleared neural tissue

nylk2016boe_thumbnailJonathan Nylk, Kaley McCluskey, Sanya Aggarwal, Javier A. Tello, and Kishan Dholakia

Biomedical Optics Exprss 7(10), 4021-4033 (2016)

doi: 10.1364/BOE.7.004021

We have investigated the effect of Airy illumination on the image quality and depth penetration of digitally scanned light-sheet microscopy in turbid neural tissue. We used Fourier analysis of images acquired using Gaussian and Airy light-sheets to assess their respective image quality versus penetration into the tissue. We observed a three-fold average improvement in image quality at 50 μm depth with the Airy light-sheet. We also used optical clearing to tune the scattering properties of the tissue and found that the improvement when using an Airy light-sheet is greater in the presence of stronger sample-induced aberrations. Finally, we used homogeneous resolution probes in these tissues to quantify absolute depth penetration in cleared samples with each beam type. The Airy light-sheet method extended depth penetration by 30% compared to a Gaussian light-sheet.