A compact light-sheet microscope for the study of the mammalian central nervous system

yang2016scirep_thumbnailZhengyi Yang, Peter Haslehurst, Suzanne Scott, Nigel Emptage, and Kishan Dholakia

Scientific Reports 6, 26317 (2016)

doi: 10.1038/srep26317

Investigation of the transient processes integral to neuronal function demands rapid and high-resolution imaging techniques over a large field of view, which cannot be achieved with conventional scanning microscopes. Here we describe a compact light sheet fluorescence microscope, featuring a 45° inverted geometry and an integrated photolysis laser, that is optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We demonstrate the utility of this design for three-dimensional morphological reconstruction, activation of a single synapse with localized photolysis, and fast imaging of neuronal Ca2+ signalling across a large field of view. The developed system opens up a host of novel applications for the neuroscience community.