Jonathan Nylk and Kishan Dholakia

Neurophotonics and Biomedical Spectroscopy (First Edition), Elsevier, ISBN: 9780323480680 (2018)

doi: 10.1016/B978-0-323-48067-3.00018-4

Light-sheet microscopy has emerged as a powerful approach for long term imaging within neuroscience and developmental biology. This chapter will cover the basics of light-sheet microscopy with an emphasis on structured light fields, namely propagation-invariant beams. The use of such fields allows the field-of-view to be maximised whist retaining high resolution. There are additional advantages of minimising photodamage.