Enhanced cell transfection using subwavelength focused optical eigenmode beams [Invited]

X. Tsampoula, M. Mazilu, T. Vettenburg, F. Gunn-Moore, and K. Dholakia

Photonics research 1(1), 42-46 (2013)

doi: 10.1364/PRJ.1.000042

We show that superoscillating light fields, created using the method of optical eigenmodes, enable more efficient multiphoton-mediated cell transfection. Chinese hamster ovary cells are transfected with a plasmid and exhibit expression of DsRed-Mito in the mitochondria. We demonstrate an efficiency improvement of 35% compared to the diffraction-limited spot. This opens up new vistas for nanoscale localized cell transfection.